Device

Part:BBa_K2761009:Design

Designed by: Carlos Javier Cordero Oropeza   Group: iGEM18_Tec-Monterrey   (2018-10-10)


Cas1-Cas2 with Flag and Ha tags


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 192
    Illegal XhoI site found at 985
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 913
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Use with BL21 only.

Source

Synthetic design.

References

Nuñez, J. K., Kranzusch, P. J., Noeske, J., Wright, A. V., Davies, C. W., & Doudna, J. A. (2014). Cas1–Cas2 complex formation mediates spacer acquisition during CRISPR–Cas adaptive immunity. Nature structural & molecular biology, 21(6), 528.